Coding
Part:BBa_J72021:Design
Designed by: Jennifer Brophy Group: Anderson Lab (2010-07-15)
{Bmll42} in BBb
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
N/A
Source
Overlap Extension PCR (Wobble Construction)
Wobble construction of Bmll42 {rbs_at_10000.HSV>}
Wobble mll42/mll43 (105, EcoRI/BamHI Sub in pBca9523-Bca1144#5 (EcoRI/BamHI, 2472+1224, L) Product is pBca9523-Bmll42 {rbs_at_10000.HSV.} ---- mll42 Forward wobble for {rbs_at_10000.HSV>} (Bmll42) CCATAGAATTCATGAGATCTCGAACACCCATATCAATATTAAAGGGAAGGTAAGCATGCAGCCGGAG mll43 Reverse wobble for {rbs_at_10000.HSV>} (Bmll42) CGTTAGGATCCGCAGTCTTCCGGGTCTTCTGGTGCGAGCTCCGGCTGCATGCTTACC